FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50198184
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG540120.10759751787036759No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA514710.10253558176526864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG42900.015.78788826
GGGAGGT280350.014.72703553
ATATAGC55500.014.5976736
GGAGGTC275650.014.5939334
ATAGGGG223650.013.92901538
AGGTCAC286750.013.8128526
GGCATAG215500.013.75808135
GGTCACT285650.013.4875977
GAGGTCA303850.013.378775
AGCTTAG221150.013.366631545
GTCACTC289750.013.2422598
ACTCTCC296750.013.17177311
TGTCGTT53500.012.91360428
GAGCTTA243650.012.29581844
AACTCTA141650.012.08597445
GCTAAGT56600.012.04462814
TGGCATA249700.012.01816834
TATAGCT67500.011.8022437
CATAGGG251250.011.73671437
CGTTCCC61500.011.59982231
Produced by FastQC (version 0.11.5)