FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56978715
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG182550.016.41732245
ATAGGGG214900.014.25580238
GGCATAG213800.014.16226635
GAGCTTA227600.013.39139744
GGGAGGT314600.012.9761993
CATAGGG240900.012.6248437
GGAGGTC319000.012.3995384
GCATAGG248250.012.29553936
TGGCATA251300.012.18168934
GTCACTC320700.011.9801018
ACTCTCC332450.011.89785611
GGTCACT327350.011.702767
GAGGTCA356200.011.2541495
TAGGGGA355750.010.60658239
CACCGAA213700.010.56518645
AGGTCAC379850.010.5126116
GCACCGA221200.010.08592544
TCACTCT395700.09.9229769
CACTCTC390750.09.91204110
AGGGGAG394350.09.81085640
Produced by FastQC (version 0.11.5)