FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54040822
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1341750.24828452831453968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT291200.024.6850641
AACGCTA333900.023.06455223
ACGCTAC335350.022.97159424
TCACGCT350000.022.5176858
CGCTACC344700.022.28323725
AAAACGC357900.021.54303221
CCGGCTT373100.021.3552762
TTCACGC375350.021.1646947
GAAAACG364400.021.12789320
ACGCTCA380500.020.97281310
AAACGCT387350.019.99803222
CGGCTTC428850.019.6670423
CACGCTC405900.019.416589
CGTGGTT399650.018.57929241
TCGTGGT423050.018.15776840
GTTTTCG410600.017.8827345
CGCTCAG459500.017.4894111
AGACCGC66000.017.386984
GCTACCT453900.017.20480226
CCTCGTG455100.017.16075338
Produced by FastQC (version 0.11.5)