FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51969146
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG140700.013.45648945
GGCATAG143000.013.10917735
ACCCGGC88800.012.2866515
ATAGGGG154250.012.28180138
CATAGGG159200.011.90017437
ATAGCTC86300.011.8622839
GAGCTTA164000.011.68322444
GCATAGG171950.011.0714736
GTCACTC247200.011.0146988
TGGCATA176100.010.79912534
GGTCACT245300.010.7988687
GGGAGGT271550.010.6825313
ACGGACA102700.010.44901418
ACTCTCC262500.010.30379811
TAGCTCT102200.010.08274640
TCACCCG117650.010.0806783
GGAGGTC277000.010.0154144
TCAGACG96450.09.986862
CGGACAC107400.09.94942213
CGGACAG109550.09.693542519
Produced by FastQC (version 0.11.5)