FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48792846
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG879010.18015140990136136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG279700.016.00715345
GGGAGGT403650.015.6518213
GGAGGTC408450.015.1155174
ATAGGGG304750.014.77558638
GGCATAG302450.014.74047935
ACTCTCC416850.014.361423511
GTCACTC423100.014.3461338
GGTCACT426950.014.1010447
GAGCTTA325100.013.87568644
TGGCATA326800.013.73955734
TGGGAGG363700.013.1812622
TCACTCT490350.012.5160959
CACTCTC479600.012.50142610
CATAGGG365550.012.33655437
GAGGTCA507050.012.3090065
AGGTCAC506600.012.2787396
CACCCGG126400.011.8778891
GCATAGG383850.011.73081736
TCTCCCC515350.011.57731913
GCTCTGT542450.011.15299122
Produced by FastQC (version 0.11.5)