FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53713181
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT916940.17071042580777332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33900.023.0970846
GAACGAT226400.021.06251345
CGGTATA49500.020.6811820
ACCGCGT224050.020.6469446
GACCGCG226000.020.4285725
AGACCGC234750.019.9642414
CCGCGTT233050.019.820637
CGCGTTC234250.019.7286998
TCGACGA40550.019.6421957
CAGACCG246050.019.1845323
TCAGACC292800.017.0127942
CGACGAG45700.016.9855848
AGAACGA283200.016.89346744
AATACGG282250.016.70807632
GCGTTCT284200.016.3245879
TACGGAG288800.016.2356534
ATACGGA291600.016.21863733
CAATACG303400.015.76582931
GTTCGAC52100.015.4169915
AAGAACG313200.015.27500443
Produced by FastQC (version 0.11.5)