FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53713181
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG133650.012.44333545
GGCATAG136100.012.25910535
ATAGGGG141600.011.95431938
CATAGGG151250.011.12251137
GAGCTTA159200.010.61940544
GTCACTC210650.010.5958698
GCATAGG159250.010.57740336
GGTCACT224750.09.9515497
GGGAGGT254500.09.92935853
GGAGGTC243150.09.7646794
AGGTCAC241750.09.7628686
GAGGTCA255350.09.6758925
ACTCTCC238000.09.63365711
TGGCATA179950.09.43145334
ACCCGGC78000.09.3300645
GCGTAGG63350.08.49587636
CGTCGAT23300.08.48184112
GGCGTAG64850.08.46043835
CGTAGGG63600.08.39206537
GTTGTCG33800.08.3731826
Produced by FastQC (version 0.11.5)