FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53101591
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG621990.11713208366958346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC112800.016.852095
TCACCCG122450.015.4565993
CGGACAC125800.014.62764413
GGTCGCC47850.014.430531543
AGCTTAG217900.014.25169345
CCGGACA135350.014.13712612
ACGGACA131500.014.07991218
CACCCGG139750.013.9108684
GTTGTCG25400.013.82830726
GGCATAG228000.013.51252135
GGAGGTC316850.013.094824
GGGAGGT355550.013.0009933
GCCCGGA153050.012.79327310
GCGTCGT55600.012.77884111
CGGACAG143500.012.71505419
TCAGACG120100.012.6300882
GTCACTC323550.012.6131828
CACGGAC146450.012.56551717
ACTCTCC331050.012.44259611
ATAGGGG255650.012.400638
Produced by FastQC (version 0.11.5)