FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50057257
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG148900.013.76774845
GGCATAG157800.012.96118735
GTTGTCG24350.012.75282326
ATAGGGG175250.011.913197538
GAGCTTA176850.011.58989544
GGGAGGT275950.011.5132943
ACCCGGC93100.011.37949855
TGGCATA183300.011.2686134
ATAGCTC88700.011.10986839
CATAGGG189650.011.02160137
GCATAGG198900.010.55429336
GTCACTC275050.010.4345578
ACGGACA99950.010.33399518
GGTCACT270300.010.3017987
TCACCCG109300.010.2519043
GGAGGTC292550.010.1337124
TAGCTCT97250.010.04053240
ACTCTCC280750.010.02398211
TCAGACG101650.09.7834972
CGGACAC109400.09.50348713
Produced by FastQC (version 0.11.5)