FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49669121
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG885450.17826971409459813No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT776270.15628824999741792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG57300.030.3894066
TCGACGA64250.027.1721767
CGACGAG65100.026.5754878
CGGTATA78700.026.44265420
ACCGCGT168950.024.9677246
GTTCGAC70600.024.887555
GACCGCG171550.024.523745
AGACCGC176300.024.182034
CCGCGTT182450.023.0832967
CAGACCG187700.022.9410633
CGCGTTC187200.022.5096028
AGTTCGA85900.021.0832984
CAGTTCG89350.020.6217353
TCAGACC225200.019.810262
GTATAGA120050.018.81525222
AACGCTA262650.018.63948623
GAACGAT232650.018.37502545
ACGCTAC266650.018.33526624
ATGACGG107150.018.3298116
TCACGCT279450.017.928838
Produced by FastQC (version 0.11.5)