FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49313768
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG913310.1852038562536937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC205800.022.55272524
AACGCTA206200.022.48558223
CGCTACC213200.021.71717625
TCACGCT236000.020.9484378
AAAACGC232950.020.70211821
GCCGGCT253100.019.8993911
GAAAACG244800.019.75512320
TTCACGC253800.019.6307547
CCGGCTT268550.019.6188052
AAACGCT243550.019.15734922
ACGCTCA266400.018.89564910
CACGCTC269200.018.348199
CGGCTTC304000.018.0484643
GTTTTCG282200.016.97918145
CGTGGTT285550.016.58113341
TCGTGGT291950.016.25216740
CTACCTC306500.015.35593927
TACCTCT309250.015.21958728
ACCGCGT62450.014.9836326
AGACCGC62850.014.9242734
Produced by FastQC (version 0.11.5)