FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53377476
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG538950.10096955502354589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG34300.023.2861086
CGACGAG39700.019.8920388
TCGACGA41400.019.8903967
CGGTATA58250.019.2736920
GTTCGAC42850.018.9553265
AACGCTA181500.016.54962223
ACGCTAC181600.016.51602224
CGCTACC185850.016.0900625
CCGGCTT215300.015.7488392
GCCGGCT200450.015.5289141
GTCCGTT41000.015.5031081
GACCGCG67500.015.2997315
TCACGCT208700.015.0927468
AAAACGC203950.014.94794621
ACCGCGT70400.014.7649516
ACGCTCA216750.014.62561510
TTCACGC215500.014.5538597
AGACCGC71650.014.4773174
GAAAACG213300.014.1659420
CTACACG67600.014.04537545
Produced by FastQC (version 0.11.5)