FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53377476
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG130050.011.12225238
AGCTTAG131800.010.33112845
GGCATAG132250.010.30635435
GTTGTCG32500.010.11372626
TAATCGG62650.09.9463624
GTAACGA58550.09.91939318
CATAGGG147700.09.41287937
TCAGACG90450.09.37752152
GGGAGGT206850.09.3529913
GGTAACG66700.09.24734417
GCATAGG153150.09.12251236
GAGCTTA158200.08.86395744
TGTCGTT38900.08.86220728
ATCGGTT75000.08.7778216
ACTCTCC211550.08.75246411
ATATAGC48800.08.7481086
ATAATCG76200.08.6500833
AATCGGT78350.08.6088045
GGAGGTC206550.08.57146554
CTAGAAT103750.08.49152851
Produced by FastQC (version 0.11.5)