FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56041061
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2672160.4768218074957574No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1112120.19844734916778253No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG828660.14786657947107748No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA794860.14183528752248284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT540950.024.30479845
AGACCGC612650.021.8594934
ACCGCGT613850.021.7634076
GACCGCG619000.021.5548555
CAGACCG634450.021.1689623
CCGCGTT631350.021.160167
CGCGTTC643250.020.7477178
AGAACGA644350.020.47440544
TCAGACC676100.020.1178132
GCGTTCT688150.019.495339
CTCAGAC786950.018.532691
AATACGG725850.018.17129332
ATACGGA732050.018.00809333
TACGGAG729250.017.98158834
CGTTCTC762200.017.85220110
CAATACG746200.017.73078531
AAGAACG745250.017.68122543
ACGGAGA764000.017.1933935
CCTCTCA788050.017.1362719
ACTCCCC789200.016.96643325
Produced by FastQC (version 0.11.5)