FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56041061
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1017690.18159720423565856No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG857260.15296998035065754No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA822930.1468441148892595No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN650560.11608631035732889No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG601280.10729275807251401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG296550.017.4223145
GTTGTCG49900.017.3168126
ATATAGC61700.015.5655026
GGCATAG337750.015.35138935
GGGAGGT477350.015.1885523
GGAGGTC458400.015.1439994
ATAGGGG348200.014.9743438
GAGCTTA354000.014.76651844
TGGCATA364800.014.3058334
TGTCGTT61150.014.02052328
GCTAAGT62700.013.92290814
TATAGCT71800.013.595357
GTCGTTC64200.013.52999629
TGGGAGG404900.013.446692
ACTCTCC529850.013.08473711
CATAGGG405450.012.87154337
CACCCGG140700.012.3561641
GCATAGG424900.012.30345136
TCGTTCC72200.012.2177830
CTGGGAG494300.012.0619471
Produced by FastQC (version 0.11.5)