FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48333225
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT77150.024.3727556
AGACCGC78400.024.2136734
GACCGCG83150.022.5869965
CGCGTTC84800.022.1471868
CCGCGTT85800.021.8631637
CAGACCG94250.020.595093
GAACGAT93000.020.34460445
AATACGG97850.019.1599932
TACGGAG98150.019.05647334
ACGCTAC92700.018.9543324
ATACGGA100850.018.72422833
AACGCTA94700.018.64905523
CGCTACC103950.016.9039425
AGAACGA116150.016.47227344
TCGGCGC85850.015.74214445
GCGTTCT121650.015.6417829
TCAGACC129050.015.6169082
TTCGGCG89650.015.29046944
AAAACGC119350.014.8906321
CAATACG130000.014.73322331
Produced by FastQC (version 0.11.5)