FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48333225
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG651820.1348596126163731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG213000.015.66981445
GGCATAG212250.015.64305135
GGAGGTC330450.014.6774414
GGGAGGT358100.014.5256353
GTCACTC325100.014.4571358
GGTCACT327750.014.4306337
GAGCTTA235250.014.32179944
ACTCTCC329750.014.24452311
ATAGGGG244250.014.04312338
GAGGTCA347050.014.0191695
TGGCATA240850.013.95472734
AGGTCAC349000.013.790856
CATAGGG245850.013.77831837
ACCCGGC91350.012.8160555
GCATAGG268050.012.59564136
TAGGGGA390850.012.01566839
TCACCCG100650.011.9916373
CACTCTC396850.011.83699310
TGGGAGG310750.011.6738392
ACGGACA96550.011.67219518
Produced by FastQC (version 0.11.5)