FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56984273
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT594290.10429017844976281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG153900.019.4256885
ACCGCGT154500.019.3661946
GAACGAT156600.019.14738845
AGACCGC156800.019.1094864
CCGCGTT165200.018.1254637
CGCGTTC169550.017.6338988
CAGACCG176650.017.1788163
AACGCTA87500.016.71378523
ACGCTAC88400.016.59452624
ATACGGA194200.015.51334633
AATACGG195850.015.35968532
CGCTACC95450.015.3221325
TACGGAG196850.015.31566434
AGAACGA201650.015.12809144
TCAGACC214000.014.6539362
GTCGGTT47950.014.6050531
CAATACG210050.014.41786931
GTCCGTT46900.014.259851
AAGAACG216350.014.00707543
CCCGTAT37100.013.8386041