FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56984273
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG151750.012.63645545
ATAGGGG156950.012.38490238
GGCATAG160900.011.99694335
CATAGGG174200.011.17141237
GTCACTC244700.010.7325388
GGGAGGT279950.010.4814443
GAGCTTA188600.010.39125644
GGTCACT248900.010.334427
GCATAGG187100.010.29295636
TGGCATA203400.09.65616234
ACTCTCC280700.09.58047911
AGGTCAC289400.09.525366
GGAGGTC289800.09.4499764
GAGGTCA311350.08.9115395
TAGGGGA302550.08.3437639
TCACTCT332850.08.1674019
GGCGTAG78150.07.859104635
CGTGGCG120800.07.8412321
CACTCTC337950.07.83771710
TTACGCG14100.07.818372235
Produced by FastQC (version 0.11.5)