FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48088567
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1038280.21590994799241991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT217050.023.62708345
AGACCGC234250.022.1868484
TTCGACG25550.021.9266876
ACCGCGT237150.021.8491946
GACCGCG239700.021.6353245
CAGACCG247550.021.130983
CCGCGTT248100.020.9028557
CGCGTTC255050.020.3243988
AGAACGA265550.019.4050544
TCAGACC290150.018.3478532
TACGGAG278750.018.20242534
AATACGG281600.018.11408632
GCGTTCT288500.018.045889
ATACGGA282600.018.01017833
TCGACGA33150.017.5783317
AAGAACG302150.017.00222243
CAATACG302450.016.99177431
CTCAGAC357100.016.5933441
ACGGAGA306650.016.58299635
CCCAATA326850.015.91609329
Produced by FastQC (version 0.11.5)