FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59734352
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6500961.0883117975398813No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA796310.13330855250593493No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG773580.12950337186214056No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC616310.10317513781684616No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG600690.10056022705327079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG175650.015.037692
TCGCGTA1758.074567E-714.16045238
GTCACTC365450.014.0559318
GGCATAG277250.013.82966335
GGAGGTC372400.013.7389864
AGGTCAC379250.013.7219196
AGCTTAG280850.013.59625545
CGTACGC62400.013.471885
GGTCACT385850.013.452147
CCGTACG62050.013.4045444
ACTCTCC403050.012.93757211
ATAGGGG317450.012.90152538
ACGGCGA172200.012.56846429
TTCGGCG66400.012.53414544
GAGCTTA305000.012.52708344
TGGCATA312850.012.38092134
CACCCGG97150.012.3802251
GAGGTCA420800.012.3068785
CGTGGCG345200.012.1588121
AACGGCG181250.012.02600428
Produced by FastQC (version 0.11.5)