FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51209606
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG990550.19343050598748993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG274800.018.12068645
GGGAGGT430450.015.7493643
GTCACTC408100.015.6726318
GGCATAG318250.015.62502235
GGTCACT415100.015.5650447
GAGGTCA429650.015.4087855
GAGCTTA327400.015.34000344
GGAGGTC427700.015.32175354
GTTGTCG28600.015.1841126
ATAGGGG332750.015.11308938
AGGTCAC441800.014.98553856
ACTCTCC438650.014.72925111
TGGCATA342750.014.54752334
TAGCGTA12250.014.14290737
TGGGAGG369100.014.0831852
TCACTCT467150.013.8746929
CATAGGG371200.013.49277337
CACTCTC494650.012.98787510
GCATAGG388800.012.91116536
CACCGAA285100.012.2885745
Produced by FastQC (version 0.11.5)