FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45836986
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT904260.19727736897884166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC189450.024.6670154
ACCGCGT188750.024.4913046
CAGACCG199150.023.601133
GACCGCG205450.022.5474975
CCGCGTT206100.022.45117
CGCGTTC208550.022.1981358
TTCGACG24100.022.028356
TCAGACC230200.020.9553132
GCGTTCT245050.018.9742979
GAACGAT245450.018.89753245
CTCAGAC304050.017.8267651
CGGTATA37500.017.456220
AATACGG269700.017.18515432
ATACGGA264350.017.1753133
TACGGAG266550.016.8646534
TCGACGA32850.016.7084377
AGAACGA280050.016.61094944
ACGGAGA284550.015.93012335
CAATACG292250.015.9053631
AACGCTA159250.015.65334223
Produced by FastQC (version 0.11.5)