FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49962293
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA787070.15753280178713974No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG543490.10878003537587837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG132100.012.70680345
GTTGTCG37200.012.6396626
ATATAGC44800.012.5558026
ATAGGGG136650.012.45018938
GGCATAG135800.012.19650335
CACCGAA353750.011.20116545
TGTCGTT42200.011.1955628
CATAGGG152500.010.97908137
GTCACTC213650.010.857728
GGGAGGT233900.010.8024973
GCATAGG158600.010.61342736
TATAGCT51650.010.5422477
GCACCGA377200.010.39789844
TGGCATA164250.010.24833934
AGGGATC168750.010.1996522
GAGCTTA164250.010.16525744
GGTCACT226800.010.1290997
GGAGGTC243800.09.9300914
TTGTCGT53050.09.66903827
ACTCTCC247750.09.59931811
Produced by FastQC (version 0.11.5)