FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53820599
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4628590.8600034347443811No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1110170.20627232335336884No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA976890.18150857072400847No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC666480.12383362734405835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT645550.034.01677745
AGAACGA766350.028.75104344
AGACCGC865350.025.5730274
CAGACCG873900.025.353713
ACCGCGT879050.025.138656
GACCGCG881600.025.0837965
AAGAACG877200.025.05501243
CCGCGTT897150.024.6490277
CGCGTTC898100.024.5853888
TCAGACC925650.024.0626222
ATACGGA922600.023.89638933
AATACGG925400.023.83208532
TACGGAG924700.023.8061734
GCGTTCT931550.023.7460389
CAATACG942650.023.39229631
ACGGAGA954450.023.0585235
CTCAGAC996350.022.9948561
CCCAATA968400.022.77104429
CGGAGAG988200.022.26570136
CACTCCC1003050.022.15379324
Produced by FastQC (version 0.11.5)