FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53820599
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA749530.13926452212098198No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG712360.1323582444706719No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG620810.11534802873524316No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG543210.1009297573964199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG172450.015.64422845
GTTGTCG46700.014.7918926
ATAGGGG188050.014.29739238
GGCATAG187800.014.05242635
TGTCGTT52450.013.38479428
GTCACTC279500.013.1976368
GGTCACT281800.013.03447157
GCTAAGT54150.012.80054514
ATATAGC57350.012.7468896
GAGCTTA215350.012.58026844
CATAGGG213650.012.5719637
TGGCATA225700.011.91221434
ACTCTCC316350.011.90358811
AGGTCAC321000.011.8563136
GGGAGGT341000.011.7858263
GCATAGG227800.011.73172636
GAGGTCA339400.011.4478025
GGAGGTC345750.011.1532324
TATAGCT69200.010.9864877
TCGTTCC64950.010.53157730
Produced by FastQC (version 0.11.5)