FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44055443
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG20150.017.5284046
GTCCGTT36500.016.2937161
GTCCGAT25300.015.7602061
GTCCCGT42200.015.0004061
GTCGGTT38400.014.8422051
CTCCGTT70950.014.6054371
CCCGTAT30800.014.55497551
CGACGAG23200.014.44827658
CTCCGAT46950.014.3464591
GTCCGGT30500.014.3288391
CTCCGGT59100.014.1796211
TCGACGA25300.013.782537
GTCCGCT44050.013.5521781
GTTCCTA90850.013.5206115
CTCGGTT86550.013.4825441
GCACTAC51700.013.23216525
CCTACTC94000.013.18746218
CTACACG68500.012.84151645
CCGGCTT139250.012.7952192
CCCCGTT53500.012.7163451
Produced by FastQC (version 0.11.5)