FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56419056
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG187700.014.06235345
GGCATAG205400.012.53662835
ATAGGGG211550.012.46646338
GGAGGTC293750.012.3799774
GTTGTCG31950.012.23442626
GGGAGGT318500.012.0597613
ACCCGGC84650.011.5955555
GAGCTTA232850.011.2681744
TGGCATA235350.011.1893834
ACGGACA89600.010.98264118
CATAGGG239800.010.936789537
TCACCCG97700.010.5758513
GCATAGG253800.010.31571636
ACTCTCC352300.010.07357911
CGGACAC97550.010.03989113
CCGGACA104500.09.69444812
GGTCACT367150.09.6523947
TAGGGGA363100.09.64312339
GTCACTC375100.09.638768
CGGCCCG111400.09.3535218