FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55720515
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1032130.1852333920459996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT178700.026.57199945
AGAACGA225750.021.19336144
AGACCGC242200.020.9019554
ACCGCGT252950.020.0212386
CAGACCG255250.019.8950443
GACCGCG256350.019.7482435
CGCGTTC260550.019.4461548
CCGCGTT260700.019.4174947
ATACGGA269100.017.79916633
AATACGG271550.017.58886732
TCAGACC293450.017.535242
TACGGAG272800.017.52476734
AAGAACG275100.017.34235443
GCGTTCT294900.017.249579
CAATACG287400.016.76757831
CCCAATA303400.015.86108529
ACGGAGA303100.015.82482635
CTCAGAC376150.015.2271721
TTCGGCG67950.015.00282644
CGGAGAG323200.014.7993336
Produced by FastQC (version 0.11.5)