FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55720515
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG143850.015.522572545
GGCATAG158350.014.21155835
ATAGGGG167700.013.49155738
GAGCTTA174900.013.05178244
GTCAGGA227700.012.9603371
CGCTCTC117300.012.47256811
CATAGGG180500.012.46723837
GCATAGG189200.011.881936
CGGGACG72350.011.80562217
ACCCGGC77900.011.7702665
GGGAGGT289350.011.7673533
TGGCATA193400.011.73833334
AGCGCTC82450.011.6108549
ATAGCTC76200.011.44428139
GGAGGTC294300.010.9279284
TAGCGTA7050.010.85268637
ACGTCTC82000.010.7457221
TCACCCG95450.010.6662543
ACGGACA89900.010.35105118
TGCGCTC55600.010.2256519
Produced by FastQC (version 0.11.5)