FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48473724
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1331010.2745838136966741No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1130820.23328515052815005No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG936360.19316857107986998No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA742240.15312213272493774No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC709220.1463101947768651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC276100.023.3671554
CAGACCG286000.022.7235833
GACCGCG294650.021.8884125
AACGCTA229500.020.94913123
ACGCTAC230750.020.85529724
TCAGACC318900.020.7604372
ACCGCGT311200.020.7315716
TTCGGCG173700.020.35873444
CGCTACC239350.020.10605625
TCGGCGC177550.019.98073445
AAAACGC250050.019.30737721
CGTGGTT255250.018.84295341
GTTTTCG257200.018.68031745
GAAAACG258700.018.62662120
AAACGCT262700.018.3615322
TCGTGGT263850.018.12663540
CTCAGAC408450.017.6292481
TTCGACG22650.016.8839236
TCACGCT298400.016.6679658
CCGCGTT406100.015.870277
Produced by FastQC (version 0.11.5)