FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48473724
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1034690.21345378787072353No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1024690.2113908145369644No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA971080.2003312144946817No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG770000.1588489466994531No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT560840.11569979645054711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG302750.016.14885945
GTCACTC394650.016.0808538
GGTCACT397950.015.9135717
AGGTCAC429100.014.9930066
GGGAGGT451250.014.8242183
ACTCTCC430050.014.7571411
TGCCCGT254050.014.55290344
GAGGTCA453550.014.45688155
GGCATAG341500.014.28887435
GGAGGTC458400.014.2645064
ATAGGGG350650.013.91651638
TCACTCT466000.013.749089
TGGCATA358850.013.66071434
GAGCTTA360500.013.62635744
CACTCTC476350.013.2424710
TGGGAGG396100.013.0083052
GTTGTCG21800.012.79660526
AGGGATC201350.012.7727582
TATCCTA121250.012.730715
CATAGGG385350.012.65744537
Produced by FastQC (version 0.11.5)