FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53449369
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT817400.15292977546657285No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG571560.10693484519901442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26250.020.5654986
GAACGAT209550.020.49921845
GACCGCG211700.020.2414475
ACCGCGT214500.020.0396756
AGACCGC217500.019.898144
CCGCGTT225300.019.0491527
CAGACCG234900.018.644123
CGCGTTC232850.018.4603238
CGGTATA39600.017.21131720
AGAACGA258750.016.68898644
TCGACGA34000.016.4070787
ACGCTAC207000.016.34422924
AACGCTA207800.016.30219523
AATACGG259750.016.22317332
TCAGACC278800.016.1761532
ATACGGA261400.016.1207133
CGACGAG31500.016.0667068
TACGGAG260000.015.94775334
CGCTACC213550.015.79088525
GCGTTCT279750.015.4699689
Produced by FastQC (version 0.11.5)