FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53449369
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC264150.011.3354234
AGCTTAG192650.011.32211645
GGGAGGT279000.011.2803823
ATAGGGG201400.011.18368338
GGCATAG199800.010.97880835
GTCACTC274350.010.4968448
GGTCACT276600.010.2640687
GTTGTCG31100.09.98337526
ACTCTCC297000.09.82608211
TTACGCG15700.09.74440435
ACCCGGC68650.09.4376595
GAGCTTA232650.09.41418344
TGGCATA239800.09.26009134
GAGGTCA338950.09.12597755
CATAGGG249850.08.97891837
AGGTCAC337400.08.9011656
GCATAGG256050.08.78745636
TAGGGGA337400.08.41665639
CACTCTC339400.08.39965110
TCACTCT355700.08.356249
Produced by FastQC (version 0.11.5)