FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61810115
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG59350.017.86071426
ATATAGC78550.015.1750916
TGTCGTT72200.014.588228
GCTAAGT78850.014.35237614
GGAGGTC360400.014.0906484
GTCGTTC76900.014.01815829
GGGAGGT383150.013.8475083
GGCATAG174800.013.78751935
ATAGGGG187000.013.704699538
CGTTCCC81650.012.78920531
GAGGTCA406000.012.7244525
TATAGCT93650.012.6324127
AGCTTAG191250.012.60021445
GGTCACT391100.012.5250197
GTCACTC392200.012.5018048
TCGTTCC84300.012.46730130
AGGTCAC414600.012.3410966
ACTCTCC404450.012.08513211
GAGCTTA211000.011.42080144
CCGGGAG251050.011.162241
Produced by FastQC (version 0.11.5)