FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57107974
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1939930.33969511858361495No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1299460.22754440562013284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG52100.026.8607986
GTTCGAC55200.025.2705695
CGACGAG55700.024.1145348
CGGTATA74700.024.0050420
GACCGCG300600.023.1201885
TCGACGA62250.022.8785217
GCCGGCT479100.022.8645971
AGACCGC308700.022.746844
ACCGCGT309900.022.4918946
CAGACCG328400.021.6629923
CCGGCTT584300.021.2490252
CCGCGTT334600.020.8583247
CGCGTTC340000.020.4871648
TTCACGC622900.020.3823977
TCACGCT632200.020.043248
TCAGACC368650.019.6959952
CGGCTTC670750.019.2065963
ACGCTCA670250.018.90538810
AACGCTA662850.018.56688523
ACGCTAC663700.018.52938324
Produced by FastQC (version 0.11.5)