FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57107974
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1018160.17828683609052565No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG772840.13532961263868334No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG639890.112049150964452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG45550.017.68293626
GGGAGGT350200.014.8203813
GGAGGTC345350.014.6235474
ATATAGC68350.013.7640166
GTCGTTC61200.013.26842229
TGTCGTT66350.012.54365128
TCGTTCC65750.012.11088830
GGTCACT411950.011.9537797
CGTTCCC68850.011.8269831
ACTCTCC415450.011.66805711
GCTAAGT69750.011.64568314
GTCACTC421800.011.621028
TGGGAGG363900.011.5587272
GGCATAG333400.011.41533835
ATAGGGG349550.011.35783438
AGCTTAG334250.011.17919345
TATAGCT89750.011.0336877
GAGGTCA466350.011.0223015
CACTCTC442550.010.8166410
CTGGGAG444850.010.518221
Produced by FastQC (version 0.11.5)