FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54833030
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1035940.18892627308758972No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG851340.15526043335558878No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT596490.10878297259881498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT239350.021.699936
AGACCGC240550.021.638854
GACCGCG242850.021.3135155
CCGCGTT249900.020.7659197
CAGACCG260000.020.1752573
CGCGTTC259850.019.9792218
GAACGAT261550.019.85682145
TCAGACC299700.018.0579152
AACGCTA268200.017.67989323
ACGCTAC270200.017.52485324
TCGGCGC113300.017.41800145
TCACGCT285550.017.3381738
CGCTACC275100.017.22975225
TTCGGCG116450.017.1598744
AGAACGA304150.017.12048544
GCGTTCT309800.016.859569
AATACGG304300.016.7981632
ATACGGA306250.016.74256533
TACGGAG308700.016.5367634
AAAACGC289450.016.4285521
Produced by FastQC (version 0.11.5)