FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54833030
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG670170.1222201289988899No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA552910.10083520826771747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG244200.014.03815145
GTCACTC353050.013.5673888
GGGAGGT374000.013.5398773
GGTCACT365450.013.0380437
GGAGGTC381050.012.9882784
ATAGGGG267950.012.94906738
GGCATAG267400.012.82235135
GAGGTCA390250.012.7801645
AGGTCAC386850.012.5610046
ACTCTCC395350.012.12823811
GAGCTTA293000.011.84601944
TGCCCGT210850.011.44397444
TGGCATA305150.011.27315134
TCACTCT434850.011.1402929
TCAGACG144150.010.9555082
CACTCTC440900.010.79869210
CATAGGG329250.010.57910937
TGGGAGG354250.010.4336862
GCATAGG346550.010.07014936
ACCCGGC81050.010.0200015
Produced by FastQC (version 0.11.5)