FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60323669
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG200500.014.5742235
AGCTTAG203700.014.37584645
ATAGGGG210850.014.10486638
GGGAGGT318800.013.1175323
GGAGGTC301600.013.0998614
CATAGGG234200.012.56401437
TGGCATA236800.012.55382434
GTCACTC311100.012.4330028
TCAGACG94850.012.4290392
GAGCTTA244300.012.28598144
GCATAGG243250.012.09657636
GGTCACT315450.012.0903637
ACTCTCC321150.011.89638911
ACCCGGC91100.011.1140415
GTTGTCG29300.010.97734226
TAGCGTA11300.010.95781637
GAGGTCA385650.010.68833355
TAGGGGA382750.010.017035539
CACTCTC394500.09.90724710
TCACCCG116700.09.850113
Produced by FastQC (version 0.11.5)