FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54184349
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT897350.1656105529661342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC185300.024.4323024
GACCGCG186850.024.157375
ACCGCGT187100.024.1248686
CAGACCG200600.022.7259453
CCGCGTT198500.022.7032997
CGCGTTC211100.021.4121488
GAACGAT227900.019.90367145
TCAGACC250600.018.7151532
ATACGGA248100.018.21023433
TACGGAG247550.018.10526834
GCGTTCT257350.017.6339769
AGAACGA258900.017.62474644
ACGCTAC118850.017.54920824
AACGCTA119750.017.37976823
AATACGG261350.017.29567732
CAATACG265450.017.19799631
TTCGGCG106950.017.06188644
AAGAACG273650.016.691243
CTCAGAC324950.016.455331
TTCGCGC24700.016.3962337
Produced by FastQC (version 0.11.5)