FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54184349
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG627630.11583234118029175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG193700.015.93438545
GGCATAG205300.015.10138535
GTCACTC315700.014.63288
ATAGGGG217950.014.431025538
GGTCACT321850.014.3463877
ACTCTCC331600.013.84888111
GGGAGGT366650.013.7884153
AGGTCAC344650.013.7367676
GAGCTTA227050.013.70327544
TGGCATA235050.013.3807334
CATAGGG237600.013.1147137
GAGGTCA384100.012.6335975
GGAGGTC383900.012.6116334
TAGCGTA10250.012.51141337
GCATAGG252700.012.35778936
TCACTCT390300.012.0495739
TAGGGGA379500.011.98714839
GCGTAGG124650.011.62385336
CGTAGGG124500.011.54746937
GGCGTAG125700.011.47306235
Produced by FastQC (version 0.11.5)