FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53129655
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1521670.2864069040162222No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT569520.10719437195667843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT369050.021.4316541
TCACGCT390250.021.3124338
CCGGCTT422500.020.5143852
TTCACGC430200.019.469327
ACGCTCA441700.019.13042810
AACGCTA430550.019.06679223
CACGCTC440250.019.0605759
ACGCTAC431950.018.97900624
CGCTACC442850.018.5067925
CGGCTTC491150.018.3710353
AAAACGC455800.018.04014421
GTCCGTT50550.017.6525631
GAAAACG467100.017.5651920
AAACGCT478250.017.26386522
GTTTTCG492700.016.48790745
CCCGTAT35950.016.4223821
CGTGGTT498350.016.38245841
TCGTGGT501300.016.36682940
ACGGTAC87800.015.84875145
CGCTCAG551850.015.54433911
Produced by FastQC (version 0.11.5)