FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53129655
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG716280.1348173632973901No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA543530.10230256529992525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG243800.014.6442145
GGGAGGT374750.014.0962013
GGAGGTC363000.013.7155954
GGCATAG263850.013.58622335
GAGGTCA371150.013.5289025
AGGTCAC370650.013.4985716
GTCACTC368500.013.4429578
ATAGGGG267750.013.4377338
GGTCACT367950.013.3102127
ACTCTCC384700.012.84177811
TGGCATA290300.012.480370534
GAGCTTA286750.012.46616844
TCACTCT404400.012.3608379
TGGGAGG326400.011.61442852
CACTCTC425700.011.56796810
TAGCGTA10250.010.97703237
CATAGGG337750.010.773416537
ACCCGGC88900.010.7548665
AGGGATC158300.010.4318952
TCACCCG96500.010.4213943
Produced by FastQC (version 0.11.5)