FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52707302
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1574740.29877074717275415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT276700.028.60645145
AGAACGA327000.024.26599144
AGACCGC337100.023.1884864
GACCGCG335350.022.9604935
ACCGCGT338400.022.8199796
CCGCGTT345750.022.3413757
CAGACCG353900.022.2593213
CGCGTTC356850.021.652748
ATACGGA374250.020.85318833
AATACGG374950.020.80132732
TCAGACC400150.020.3948632
AAGAACG392050.020.25175543
TACGGAG385100.020.1802134
GCGTTCT400700.019.3629
CAATACG409850.019.34296831
ACGGAGA412050.018.9594935
CTCAGAC475500.018.4632871
CGGAGAG426600.018.191236
CCCAATA444800.018.13910329
TTCGGCG91050.017.4299444
Produced by FastQC (version 0.11.5)