FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52707302
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC97650.013.7965745
AGCTTAG157650.013.39661745
TCACCCG112800.012.8217423
GGCATAG170900.012.37760935
GTCACTC250550.011.9653338
ATAGGGG185350.011.80046438
GAGCTTA190800.011.35982244
ATAGCTC111250.011.24605539
ACGGACA127800.011.19752318
CGGACAG117600.011.13542619
GGTCACT268950.011.104477
CGGACAC123050.010.97040913
CCGGACA128600.010.96796812
ACTCTCC275950.010.89717611
GGGAGGT327800.010.8896293
CATAGGG199000.010.83162737
TGGCATA202400.010.68510334
TCAGACG103550.010.6654252
GCATAGG207150.010.40626436
GGAGGTC300150.010.3480854
Produced by FastQC (version 0.11.5)