FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46844164
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG741870.15836978113218117No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT504400.10767616644839687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33100.026.1702386
ACCGCGT106700.024.9245916
AACGCTA163350.024.82045223
ACGCTAC163750.024.73234224
GACCGCG108900.024.4210625
AGACCGC109650.024.1924674
CGCTACC170550.023.71985225
CCGCGTT115850.022.8976977
CGCGTTC118600.022.4047078
AAAACGC181800.022.3139221
CAGACCG121800.022.0193213
CGGTATA52750.021.53986420
TCGACGA41250.021.3814017
AAACGCT193900.021.02589222
GAAAACG193800.020.87420520
CGACGAG41300.020.7562528
TCACGCT213800.020.594788
GAACGAT131950.020.20707345
AATACGG136250.019.5692432
ATACGGA137550.019.4497233
Produced by FastQC (version 0.11.5)