FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45918957
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG655310.1427101229672965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG182700.018.00078445
ATAGGGG201650.016.61041538
GGCATAG201500.016.18790435
GGGAGGT256100.015.4220183
GTCACTC251100.015.17421158
GAGCTTA222000.014.88507244
CATAGGG223800.014.66514337
GGTCACT260400.014.537147
GGAGGTC270000.014.5118524
GCATAGG229350.014.3397536
AGGTCAC274750.014.1545566
TGGCATA240000.013.69427534
ACTCTCC277750.013.66145711
GAGGTCA291700.013.5403085
TCACTCT302400.012.7190949
CACTCTC300800.012.457540510
TAGGGGA307200.012.43362139
TGGGAGG298400.011.7723352
TCTCCCC344200.011.05015713
AGGGGAG352550.011.00013840
Produced by FastQC (version 0.11.5)