FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51117171
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT590410.11550130581365703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26350.022.8838086
ACCGCGT138100.019.9905626
GACCGCG138500.019.916665
AGACCGC143800.019.2765524
GAACGAT144200.018.95760345
CCGCGTT149200.018.4430087
TCGACGA34750.017.6111727
CAGACCG161050.017.3655433
CGGTATA48600.017.22182320
CGCGTTC161850.017.1127388
CGACGAG36700.016.1849738
AATACGG175900.015.72023132
AGAACGA179050.015.49391644
ATACGGA179950.015.39143433
AACGCTA142050.015.12636623
ACGCTAC143800.014.95792824
TACGGAG184050.014.93854334
CCCGTAT36300.014.8873521
TCAGACC192050.014.8319192
CAATACG192850.014.37354731
Produced by FastQC (version 0.11.5)