FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51117171
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3958710.7744383976178963No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG624400.12215073482841998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG178250.017.35643645
ATAGGGG193000.016.0967338
GGCATAG193800.016.00853535
GAGCTTA209050.014.95010244
CATAGGG219700.014.2022237
GTCACTC298500.013.9535618
GGGAGGT324750.013.771493
TGGCATA229450.013.61930334
GGTCACT309800.013.4011547
GCATAGG233950.013.33752436
GGAGGTC327900.013.2553874
ACTCTCC318750.013.07879311
GAGGTCA346000.012.6650555
AGGTCAC341800.012.6147216
TAGGGGA345000.011.89312239
GTTGTCG35800.011.81569326
TCACTCT367050.011.6038149
CACTCTC369900.011.23357810
ATATAGC46700.010.9386376
TGGGAGG322650.010.6033442
Produced by FastQC (version 0.11.5)